A woman breaking into a breathalyzer with another woman standing next to her.
Alaina Holt, a VCU doctoral student, demonstrates the researchers' experiment in the VCU Center for the Study of Tobacco Products, along with Akansha Anbil, a research assistant in the center. (Photo by Thomas Kojcsich, University Marketing.)

Can vaping cause you to fail a sobriety test?

While ethanol is often a hidden ingredient in e-liquids, a new study finds vaping won’t trigger a false positive sobriety test – but only if police employ a proper waiting period.

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Five years ago, researchers led by Virginia Commonwealth University forensic science professor Michelle Peace, Ph.D., revealed that e-liquids — the flavored nicotine solution used in e-cigarettes — frequently contain ethanol, or alcohol, as an unlisted ingredient.

That hidden presence of ethanol in e-liquids raised an important question for public safety and law enforcement: Could unknowingly vaping alcohol cause a person who hadn’t been drinking to fail a sobriety test?

To answer that question, Peace and co-principal investigator Alison Breland, Ph.D., an associate research professor in Department of Psychology in the College of Humanities and Sciences and Center for the Study of Tobacco Products at VCU, launched a human clinical study in collaboration with the Virginia Department of Forensic Science and VCU Police Department.

The researchers had 13 participants vape e-liquids with either a concentration of 20% ethanol or 0% ethanol.

Before and after vaping, Lt. Edgar Greer of VCU Police administered preliminary and evidentiary breath tests and a standardized field sobriety test — including a horizontal gaze nystagmus test, walking heel-to-toe in a straight line, and standing on one foot — that replicates law enforcement officers’ roadside procedures in suspected DUI cases.

They found that breath ethanol concentrations were detectable by the preliminary breath test immediately (less than one minute) after vaping an e-liquid containing 20% ethanol.

However, when the police officer waited 15 to 20 minutes — the standard waiting period in a DUI roadside stop — after the person vaped an e-liquid with 20% ethanol, the preliminary breath test had no result, as they predicted. The researchers also found that vaping ethanol did not impact the standardized field sobriety test, and the evidentiary breath test was not affected by vaping ethanol at all.

A woman leaning next to a woman sitting down
Michelle Peace, Ph.D., a forensic science professor, and Alison Breland, Ph.D., an associate research professor in Department of Psychology in the College of Humanities and Sciences and Center for the Study of Tobacco Products at VCU. (Photo by Thomas Kojcsich, University Marketing.)

“We’re reporting negative results and we’re thrilled about that,” Peace said. “When we ran the study, we found that the 15-to-20-minute wait period is solid. We needed to get that into the literature because there are attorneys who are trying to use vaping as a reason to get somebody out of a DUI charge. What we’re now able to say is vaping does not impact the preliminary breath test if a state or jurisdiction is maintaining that 15-to-20-minute wait period.”

Why is this important? “Waiting 15 to 20 minutes gives ethanol time to reabsorb and disappear from the mouth. The waiting period is so important because e-liquids are sticky and will often remain in the mouth shortly after vaping. We were afraid that sticky nature may make it harder for ethanol to disappear from the mouth,” Peace said.

The researchers’ findings will have an impact beyond law enforcement. Alaina Holt, a doctoral student in the Integrative Life Sciences Program at VCU who is working with Peace, explained that the findings should be considered in other contexts of alcohol testing, including workplace screening tests and vehicle ignition interlock systems.

“Some agencies and industries use a preliminary breath test device to indicate alcohol consumption. If they don't adhere to a 15-to-20-minute wait period, then somebody who has been vaping continuously may test positive in that scenario,” Holt said. Peace noted, “It’s important because people’s lives are on the line at that point.”

A collaborative, multidisciplinary study

The findings of the study, “Impact of Vaping Ethanol on the Roadside Field Sobriety Test in a Clinical Setting,” will be presented this week at the annual meeting for the International Association of Forensic Toxicologists in France. It was funded by the National Institute of Justice in the Office of Justice Programs in the U.S. Department of Justice for a project titled “Through the E-Cigarette Looking Glass: The Impact of Vaping Ethanol on DUI Investigations.”

The NIJ awarded Peace and her collaborators two grants totaling roughly $1.8 million in 2018 and 2019 to study the implications of vaping ethanol.

The experiments are being conducted at the Center for the Study of Tobacco Products, which brings together a multidisciplinary group of faculty and staff from VCU, American University of Beirut, Johns Hopkins University, the University of Arkansas for Medical Sciences, the Oklahoma Health Sciences Center and the University of Southern California, as well as several other U.S. and international universities and organizations to focus on the regulation of tobacco products.

The preliminary and evidentiary breath tests equipment used in the study was provided by the Virginia Department of Forensic Science.

Akansha Anbil, a research assistant in the Center for the Study of Tobacco Products, is primary runner of the ethanol study. She conducts the experiments in a room with a specialized ventilation system that removes the e-cigarette-produced vapor while a participant vapes from an e-cigarette hooked up to a monitoring system.

A man holding a camera up to a woman's eyes
Lt. Edgar Greer of VCU Police administers a horizontal gaze nystagmus test, part of the standardized field sobriety test, on doctoral student Alaina Holt as part of the experiment. (Photo by Thomas Kojcsich, University Marketing.)

“We’re using this [e-cigarette device] because we can put a liquid into it that has either 20% ethanol or none. There's no nicotine in it,” Breland said, demonstrating how the process works. “And while they're vaping, this hardware and software designed by our colleagues at the American University of Beirut measures exactly how they're puffing. We can measure the length of their puff, the puff volume. We're asking everybody to take four-second puffs to try to be similar to how many e-cigarette users puff.”

Breland said the researchers’ findings raise questions about whether the FDA should consider regulating ethanol in e-liquids.

“We’re not trying to answer this question, but should ethanol in e-liquids be something that FDA is thinking about?” she said. “Because they do have the power to regulate components of e-liquid.”

A deeper dive into the impacts of vaping ethanol

For the team’s second project, which is currently underway, participants will be asked not only to vape e-cigarettes that might contain ethanol but also drink beverages that might contain alcohol.

The researchers will again have participants complete the standardized field sobriety test and breath test, and they will also collect blood, urine and saliva. They plan to evaluate and analyze six ethanol metabolites in the participants’ fluids.

Their goal is to better understand what happens within the body when someone vapes ethanol and what happens when someone vapes ethanol in combination with drinking alcohol.

“When you drink alcohol, there is a very predictable and known rate of absorption, distribution, metabolism and elimination in the body,” Peace said. “There are limited studies of the impact of inhaling ethanol.”

A man and woman standing in a room.
Lt. Edgar Greer of VCU Police administers a standardized field sobriety test on doctoral student Alaina Holt. The test is administered before and after participants vape in the experiment. (Photo by Thomas Kojcsich, University Marketing.)

Tests for alcohol metabolites in bodily fluids are commonly used to detect whether someone has consumed alcohol in some broad timeframe. Depending on the nature of the drug test, testing positive for ethanol may mean someone is fired, or a child is taken away, or someone violates probation/parole. The researchers’ new study could inform this process by providing information about whether vaping ethanol can result in a failed drug test.

“There are people testing positive for ethanol metabolites who will proclaim that they do not drink, but they vape,” Peace said.

The researchers’ goal is to develop a unified method for the metabolites, allowing them to address questions such as: Is the person a long-term consumer of ethanol? Or have they consumed ethanol in the past couple hours? And does vaping ethanol change those expectations?

“We know the standard activity of ethanol in the body. Does vaping ethanol change that known activity?” Peace said. “This is becoming a really pressing question that a lot of people are asking for the results on.”